The following prerequisites will be installed with the application:
- Microsoft .NET Framework 4.5.1 (x86 and x64)
Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite.
In this tutorial, we show the analysis of intact protein spectra through deconvolution, deisotoping, and searching with ProSight Lite, a free, vendor-agnostic tool for the analysis of top-down mass spectrometry data. We illustrate with two examples of intact protein fragmentation spectra and discuss the iterative use of the software to characterize proteoforms and discover the sites of post-translational modifications.
Click here to view the tutorial on PubMed.
This research is supported by P30DA018310 from the National Institute on Drug Abuse and R01GM067193 from the National Institute of General Medical Sciences.