ProSight Lite

ProSight Lite is a free Windows application for matching a single candidate protein sequence and its modifications against a set of mass spectrometric observations.

The following prerequisites will be installed with the application:
  • Microsoft .NET Framework 4.5.1 (x86 and x64)
ProSight Lite has been published in Proteomics!
Click here to check out our technical brief.
ProSight Lite was presented at the 2014 Annual Conference of the American Society for Mass Spectrometry.
Click here to download a PDF of the poster.
Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite.
In this tutorial, we show the analysis of intact protein spectra through deconvolution, deisotoping, and searching with ProSight Lite, a free, vendor-agnostic tool for the analysis of top-down mass spectrometry data. We illustrate with two examples of intact protein fragmentation spectra and discuss the iterative use of the software to characterize proteoforms and discover the sites of post-translational modifications.
Click here to view the tutorial on PubMed.
This research is supported by P30DA018310 from the National Institute on Drug Abuse and R01GM067193 from the National Institute of General Medical Sciences.